SYSY/mCLING ATTO 488标记为-710 006AT3/5 nmol/710 006AT3
市场价：
¥7100.00
美元价：
4260.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
Cat. No.: 710 006AT3
Amount: 5 nmol
Price:355.00€
| Cat.No.710006AT3 | 5nmol mCLING labeled with ATTO®488 in 100µl PBS (lyophilized). For reconstitution add 100µl H2O, then aliquot and store at -80°C until use.Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye. |
| Applications | ICC:1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml)galleryIHC:1 : 25 up to 1 : 50 (1 - 2 nmol/ml) |
| Label | ATTO 488 |
| Remarks | Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4%paraformaldehyde (PFA) and 0.2% glutaraldehyde is strongly advised. For detailed protocols see ReveloNH&RizzoliSO,2016. |
| Data sheet | 710_006at3.pdf |
References for mCLING-ATTO 488-labeled - 710006AT3
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.WiedemanJ, Mensa-WilmotKPloS one (2018) 135: e0197541. 710006AT3 ICC, FACS
Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis.ShinW, GeL, ArpinoG, VillarrealSA, HamidE, LiuH, ZhaoWD, WenPJ, ChiangHC, WuLGCell (2018) : . 710006AT3 ICC
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.WiedemanJ, Mensa-WilmotKPloS one (2018) 135: e0197541. 710006AT3 ICC, FACS
Otoferlin Depletion Results in Abnormal Synaptic Ribbons and Altered Intracellular Calcium Levels in Zebrafish.ManchandaA, ChatterjeeP, BonventreJA, HaggardDE, KindtKS, TanguayRL, JohnsonCPScientific reports (2019) 91: 14273. 710006AT3 UPTAKE; tested species: zebrafish
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