SYSY/抗兔IgG Fab-702 rbCpH/50µg/702 rbCpH
市场价：
¥9000.00
美元价：
5400.00
产品分类：
蛋白纯化试剂盒
公司分类：
Protein_purification_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Cat. No.: 702-rbCpH
Amount: 50 µg
Price:450.00€
| Cat.No.702-rbCpH | 50 µg affinity purified Fab fragments, lyophilized. Rabbit serum albumin was added for stabilization. For reconstitution add 50µl H2O to get a 1mg/ml solution of antibody in PBS.Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt!Avoid bright light to minimize photo bleeching of the fluorescent dye. |
| Applications | ICC:yes (see remarks) gallery |
| Label | CypHer5E |
| Reactivity | Reacts with: rabbit.Other species not tested yet. |
| Remarks | Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency. The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.ICC:Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µl for 30 min at RT in the dark.Adjust the antibody to the desired dilution and immediately use it for your internalization assay. |
| Data sheet | 702-rbcph.pdf |
品牌介绍
SYSY我们的“隔壁”合作伙伴公司NanoTag Biotechnologies专门研究基于单域抗体的亲和试剂（“标签”），用于生物化学和基于荧光的应用。他们提供亲和树脂，标签特异性FluoTags和用于各种免疫测定（ICC，IHC，IHC-P，WB等）的辅助试剂。Synaptic Systems在全球范围内分销NanoTag产品组合。可以轻松设置定制项目来开发定制的单域抗体。在临床神经科学系谢汉内洛尔·埃伦赖希教授在哥廷根和突触系统的MPI为实验医学已经成功地进行了多次联合研究项目。我们非常感谢他们在实验设置中验证抗体的贡献。在分子神经生物学系尼尔斯博泽教授在哥廷根的MPI用于实验医学已经突触系统自成立以来，紧密的合作伙伴。我们的几种抗体是在他们的实验室中开发的。位于莱比锡的Paul Flechsig脑科学研究所的 WolfgangHärtig教授实验室是我们最重要的外部验证合作伙伴之一，在组织学应用方面拥有丰富的专业知识。
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